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AP Biology-pGLO Transformation Lab

Our class used this procedure to transform bacteria with a gene that codes for Green Fluorescent Protien (GFP). The real life source of this gene is the bioluminescent jellyfish Aequorea victoria. GFP causes the jellyfish to fluoresce and glow in the dark. Following the transformation procedure, the bacteria express their newly acquired jellyfish gene and produce the fluorescent protein, which causes them to glow a brilliant green color under ultraviolent light. Likewise, this lab was performed on a smaller scale with Bio-rad’s unique pGLO plasmid, which encodes the gene for GFP, a gene for resistance to the antibiotic ampicillin, and incorporates a special gene regulation system, which can be was used to control expression of the fluorescent protein in transformed cells.  In this case, the students inserted some new DNA, the pGLO plasmid, into the bacteria E. coli cells was genetically engineered to carry the GFP and a gene (bla) that codes for a protein that gives the bacteria resistance to an antibiotic. The genetically engineered plasmid was then used to genetically transform bacteria to give E. coli this new trait, depicting the validity of transformation. The students’ success rates were more than successful with an ecstatic teacher. Great job class!





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